Primer

Part:BBa_K496000:Design

Designed by: Laurynas VANAGAS   Group: iGEM10_HokkaidoU_Japan   (2010-08-25)


Forward primer for visualizing restriction enzyme digestion.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction enzyme digestion cut offs must be big enough to view under UV light. When 2 restriction enzymes are used prefix and suffix cut offs must be distinguishable. Tried to make primer sites universal for every plasmid.


Source

Designed after pSB1C3 pSB1A3 pSB1T3 plasmid universal primer sites.

References